Citazione:

Grazie a te che hai permesso di sviscerare un po' di più l'argomento.
Aggiornerò questo post ogni tanto. Quando vuoi dacci un occhio le tue conoscenze mi potrebbero tornare utili.


Citazione:

L'unica cosa che ho trovato è presente nella richiesta di brevetto ed è la seguente:

a) Differentiation into Neuroblasts/Neurons in Suspension

To the mesenchymal stem cells suspended in physiological solution 6 μl/ml of neuronal differentiation solution are added.

The solution obtained is delicately resuspended and maintained at 37° C. for a period comprised between 20 minutes and 2 hours, preferably between 40 minutes and 1 hour and 30 minutes, in function of the maturation state (neuroblasts-neurons) desired.

At two hours mature neurons with dendrites and axons completely formed and functional are present (see FIGS. 1 and 2 above).

The solution for neuronal differentiation is composed thusly:

10 ml of 98% ethanol
10 mg of retinoic acid.

The relative quantities indicated above are to be considered exclusively as a practical indication for obtaining a differentiation solution having a retinoic acid concentration of 3×10−3 M.

The neuronal differentiation solution is agitated to dissolve the retinoic acid and maintained refrigerated at 4° C.

b) Differentiation into Neuroblasts/Neurons in Adhesion

Six microlitres per millilitre (6 μl/ml) of the neuronal differentiation solution prepared just before use (maximum 1 hour) and stored at +4° C. in the dark is added to the mesenchymal stem cells adherent to the wall of a culture flask.

The flask is placed in an incubator for a period comprised between 20 minutes and 2 hours, preferably between 40 minutes and 1 hour and 30 minutes, in function of the maturation state (neuroblasts-neurons) desired.

At two hours mature neurons are present with completely formed and functional dendrites and axons, the morphology of which can be appreciated (see FIGS. 3 and 4) and on which it is possible to conduct immunohistochemical and electrophysical tests.

The solution for neuronal differentiation is composed thusly:

10 ml of 98% ethanol
10 mg of retinoic acid.

The relative quantities indicated above are to be considered exclusively a practical indication for obtaining a differentiation solution having a retinoic acid concentration of 3×10−3 M.

Brevetto

ovviamente non ti so dire se è sufficiente come spiegazione per un professore, anche se credo fortemente di no, per tornare al discorso più sopra.